请教:VSV△G*G是什么意思?师兄们,师姐们,虫虫们
2021-02-25 08:19
VSVΔG*-G is the recombinant VSV which was generated by reverse genetics as described previously (Takada et al., 1997⇓ ) and kindly provided by M. A. Whitt (University of Tennessee, TN, USA). The virus was derived from a full-length cDNA clone of the VSV genome (Indiana serotype) in which the coding region of the G protein was replaced by the coding region of a modified version of the GFP gene (ΔG*) and complemented with the VSV G protein. VSVΔG*-G was grown and harvested by infecting 293T cells that had been transfected previously with pCAG-VSVG.
看得懂吧?
ΔG,表示VSV这个病毒中吸附并侵入细胞的G蛋白缺失,这个没问题
G*,表示一种修饰的GFP,估计就是EGFP,我猜,你可以去看这里引用的paper
然后把这两件事情,就是用GFP基因替代VSV中的G基因,叫做ΔG*
后面的事情就是按你理解的来了,这样一个病毒基因组是没有办法产生有功能的病毒颗粒的,所以单独再表达一个VSV的G蛋白,表达出来的G蛋白参与病毒颗粒的组装,以及下一轮感染,这里可能都谈不上“假类”,HIV用VSV-G的coating protein叫pseudotype,这里还是VSV,还是这一类。这是病毒生物安全控制得一种常规办法,就是所谓的一次性病毒。
整个事情,就是你看到的VSV-ΔG*-G
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